Dissection of key factors correlating with H5N1 avian influenza virus driven inflammatory lung injury of chicken identified by single-cell analysis.

Chicken lung is an important target organ of avian influenza virus (AIV) infection, and different pathogenic virus strains lead to opposite prognosis. Using a single-cell RNA sequencing (scRNA-seq) assay, we systematically and sequentially analyzed the transcriptome of 16 cell types (19 clusters) in the lung tissue of chickens infected with H5N1 highly pathogenic avian influenza virus (HPAIV) and H9N2 low pathogenic avian influenza virus (LPAIV), respectively. Notably, we developed a valuable catalog of marker genes for these cell types. Compared to H9N2 AIV infection, H5N1 AIV infection induced extensive virus replication and the immune reaction across most cell types simultaneously. More importantly, we propose that infiltrating inflammatory macrophages (clusters 0, 1, and 14) with massive viral replication, pro-inflammatory cytokines (IFN-ß, IL1ß, IL6 and IL8), and emerging interaction of various cell populations through CCL4, CCL19 and CXCL13, potentially contributed to the H5N1 AIV driven inflammatory lung injury. Our data revealed complex but distinct immune response landscapes in the lung tissue of chickens after H5N1 and H9N2 AIV infection, and deciphered the potential mechanisms underlying AIV-driven inflammatory reactions in chicken. Furthermore, this article provides a rich database for the molecular basis of different cell-type responses to AIV infection.

TiO2/CdS composite photocathode improves the performance and degradation of wastewater in microbial fuel cells.

Optimization of dye decolourization for wastewater and power production are explored in dual-chamber microbial fuel cells (MFCs) with TiO2/CdS photocathodes. The rapid reduction of azo dye methylene blue (MB) and power production were enhanced with TiO2/CdS photocathode under illumination. The analysis of electrochemical impedance spectra indicated that the photocatalysis of TiO2/CdS accelerated the electron transfer process of photoelectrode reduction. Moreover, the UV-visible light spectrophotometer showed that the maximum degradation of the MFCs was 98.25%, which illustrated that MB may be cleaved by photoelectrons generated by light irradiation on the illuminated TiO2/CdS photocathode. Finally, the power production of MFCs in this work promoted reductive decolourization of the dye MB solution.

Enhanced performance and degradation of wastewater in microbial fuel cells using titanium dioxide nanowire photocathodes.

This paper explores the decolorization of dye wastewaters and electricity generation using dual-chamber microbial fuel cells (MFCs) with titanium dioxide nanowire (TiO2 NW) photocathodes. TiO2 NW cathodes under ultraviolet light are observed to enhance the reduction of azo dye Active Red 30 (AR 30) and electricity generation. The analysis of electrochemical impedance spectra (EIS) indicates acceleration of the electron transfer processes of photoelectrode reduction by the photocatalysis of TiO2 NWs, with polarization resistance of the photocathode being 10.45 Ω under light irradiation from 294 Ω in the dark. Ultraviolet-visible light spectroscopy shows that the maximum degradation of the MFCs is 78.1%; the azo bond of AR 30 may be cleaved by photoelectrons generated by light irradiation of the illuminated TiO2 NW photocathode. The electricity produced by microbial fuel cells (MFCs) is expected to enhance the reductive decolorization of the azo dye AR 30 solution.

Synthesis and Adsorption Performance of a Hierarchical Micro-Mesoporous Carbon for Toluene Removal under Ambient Conditions.

Ordered mesoporous carbons (OMCs) were synthesized in this study through a soft template method and then activated by employing different mass ratios of KOH/OMCs to obtain KOH-activated ordered mesoporous carbons (KOMCs) with hierarchical pore structures. To verify the adsorption capacity, the KOMCs have been subjected to toluene emission-reduction experiments. The KOMCs were characterized by TEM, XRD, N2 adsorption-desorption isotherms, and Raman spectroscopy. The pore structure of OMCs was found to be effectively optimized by the activation with KOH, with the BET-area and total pore volume values reaching as high as 2661 m2 g-1 and 2.14 cm3 g-1 respectively. Then, the dynamic adsorption capacity of toluene on KOMCs was investigated via breakthrough curves, which can be well described by the Yoon and Nelson (Y-N) model. The dynamic adsorption capacities of toluene exhibit the following order: OMC < KOMC-1 < KOMC-5 < KOMC-3. The sample activated by KOH/OMC with a mass ratio of 3:1 (KOMC-3) demonstrated the highest toluene adsorption capacity of 355.67 mg g-1, three times higher in comparison with the untreated carbon (104.61 mg g-1). The modified hierarchical porous carbons also exhibited good recyclability. The KOMCs with rich pore structure, high toluene adsorption capacity, and superior reusability thus display a huge potential for volatile organic compound (VOC) elimination.

Preparation of Hollow Flower-Like Microspherical ß-Bi2O3/BiOCl Heterojunction and High Photocatalytic Property for Tetracycline Hydrochloride Degradation.

Tetracycline cannot be effectively degraded in wastewater treatment. Therefore, the development of excellent photocatalysts is of significant importance for environmental protection. In this study, a ß-Bi2O3/BiOCl heterojunction photocatalyst with hollow flower-like microspheres was successfully synthesized by the in-situ reaction of HCl and ß-Bi2O3 hollow spheres. The prepared samples are characterized by Scanning electron microscopy, Transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, N2 physical adsorption, UV-vis diffuse reflectance spectroscopy, and Photoluminescence. Then, research on the photocatalytic performance for the degradation of tetracycline hydrochloride was conducted. The results show that the photocatalytic performance of the ß-Bi2O3/BiOCl composite is significantly better than the ß-Bi2O3 and BiOCl. The increase in photocatalytic activity is due to the formation of a heterojunction between ß-Bi2O3 and BiOCl, which effectively promotes the separation of photogenerated electron-hole pairs. Additionally, the heterojunction nanocomposite demonstrated the outstanding photocatalytic stability after five cycles, which indicates that the material can be used for water environment purification. This paper provides assistance for studying the photocatalytic mechanism of heterojunction photocatalytic materials.